Data for Publication: Iontronic Click-to-Release Enables Electrically Controlled Delivery of Drugs and Biomolecules Beyond Charge and Size Limitations

Context and methodology

This dataset was generated within a research project investigating the combination of iontronic delivery systems with bioorthogonal click-to-release chemistry to enable electronically controlled release of drugs and biomolecules. The corresponding peer-reviewed publication describes the scientific background, experimental design, and conclusions in detail, while the Supporting Information provides extended methodological and analytical context. The dataset contains selected, curated data underlying the published figures, as well as raw NMR data supporting the structural characterization of all synthesized compounds.

The purpose of this dataset is to document the experimental data underlying the key findings reported in the associated publication. It supports results related to iontronic transport of charged tetrazines, electronically triggered click-to-release reactions, controlled release of both small-molecule and macromolecular payloads, and the structural characterization of all synthesized compounds by NMR spectroscopy.

The data were generated using established experimental techniques, including fabrication and operation of iontronic pumps, electrochemical measurements, fluorescence-based quantification assays, chromatographic analyses (HPLC/LC-MS), NMR spectroscopy for compound characterization, protein analysis by SDS–PAGE, and cell-based viability assays. Detailed experimental procedures and conditions are described in the accompanying publication and Supporting Information.

Technical details

The dataset consists of a structured Excel file containing selected quantitative data used for the generation of all figures presented in the associated manuscript. Individual worksheets correspond to specific figures or experimental sections, enabling straightforward mapping between the dataset and the published results.

In addition, the dataset includes raw NMR spectroscopy data (FID files) for all compounds reported in the associated publication. The NMR data are provided as obtained directly after measurement and allow independent reprocessing using commonly used NMR software such as Mnova or TopSpin. Files and folders are named according to the original experiments.

No proprietary data formats are used. The dataset can be accessed and analyzed using standard spreadsheet software (e.g., Microsoft Excel or equivalent open-source alternatives) and standard NMR processing software, respectively.

All additional experimental details, compound characterization, kinetic analyses, and supplementary datasets required for full interpretation of the data are provided in the Supporting Information associated with the publication.

Assignment of NMR data to specific compounds and compound numbers is described in the associated publication and Supporting Information.

¹H and ¹³C NMR spectra were recorded on Bruker Avance UltraShield 400 MHz and Bruker Ascend 600 MHz spectrometers at 20 °C.

Further details

The data are suitable for reuse in comparative studies, methodological benchmarking, meta-analyses, or educational contexts, provided that appropriate attribution to the original publication is given.