Published December 2, 2022 | Version 1.0.0
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SMLM datasets of the T cell receptor (TCR) on murine T cells on non-activating bilayers (label: CD3zeta-PS-CFP2 and H57-AF647)

Creators

  • 1. TU Wien, Vienna, Austria

Contributors

  • 1. TU Wien
  • 2. Technische Universität Wien
  • 3. Medical University of Vienna

Description

Single molecule localization microscopy images recorded by Benedikt Rossboth (BR) and Florian Baumgart (FB) for Fig. 2b and Fig. 3b in Rossboth et al. 2018 Nature Immunology.

CD3zeta-PS-CFP2 PALM data: recorded 2016/11/21, 2016/11/15, 2016/09/28 by BR

H57-Alexa Fluor 647 dSTORM data: recorded on 2017/01/23 and 2017/01/24 by BR and FB

all recordings on fixed T-cells attached to non-activating bilayers (see publication for more details).

File format: SPE (TIFF). 

Loading of files: 

1. FIJI (supports SPE format)
2. Python:  3.1. pims.open or 
                    3.2. https://github.com/schuetzgroup/sdt-python (documentation: https://schuetzgroup.github.io/sdt-python/io.html)
3. Matlab: function can be provided upon request

Files

CD3zeta-PS-CFP2_non-activating_Fig3b_2016_09_28 zeta PALM ICAM.zip

Files (19.1 GiB)

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600.4 MiBPreview Download
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3.0 MiBPreview Download

Additional details

Related works

Is part of
Journal Article: 10.1038/s41590-018-0162-7 (DOI)

Funding

FWF Austrian Science Fund
In Vivo Nanopatterning von Membranproteinen P26337-B21
FWF Austrian Science Fund
Einzelmolekül-Plattform für Protein Interaktions Analysen P 25730-B21
FWF Austrian Science Fund
3D-Nanoskopie der Immunologischen Synapse P 30214-N36
FWF Austrian Science Fund
Palmitoylierung in der frühen T-Zell Signaltransduktion P27941-B28
FWF Austrian Science Fund
Temperaturwahrnehmung durch Nanostrukturen in Zellmembranen I1953-B20

Dates

Created
2016-09-28
CD3zeta-PS-CFP2
Created
2017-01-23/2017-01-24
H57-AF647
Created
2016-11-15
CD3zeta-PS-CFP2
Created
2016-11-21
CD3zeta-PS-CFP2