Data related to publication "Reproducibility crisis in isothermal amplification: Lessons from benchmarking LAMP assays"

General Information: This data is related to the publication "Reproducibility crisis in isothermal amplification: Lessons from benchmarking LAMP assays" by Piglmann L., Campostrini L., Sommer R., Kirschner A., Krska R., Farnleitner A.H., Kolm C. and Reischer G.H. (2025) submitted to Applied and Environmental Microbiology. The raw data of the analysed LAMP assays are split up into four files: "File 1: First Assessment", "File 2: Specificity Experiments", "File 3: Sensitivity Experiments", "File 4: LOD Experiments". 

In File 1 the raw data of the comparative assessment of nine LAMP assays via fluorescence monitoring are summarized. To this end, each assay was tested with a 1:10 dilution series of genomic DNA from P. aeruginosa type strain ATCC 10145, ranging from 100.000 to 100 genomic copies per reaction (gc/rxt), analysed in triplicates.

In File 2 the raw data of the specificity experiments of seven LAMP assays via fluorescence monitoring are summarized. For these experiments all LAMP assays were assessed with 19 non-target strains with a genomic DNA concentration of 1.000 gc/rxt and in triplicates. 

In File 3 the raw data of the sensivitiy assessment of three LAMP assays via fluorescence monitoring using 12 target strains (6 clinical isolates and 6 environmental isolates) are summarized. The strains were analysed with a genomic DNA concentration of 1.000 gc/rxt and in triplicates.

In File 4 the raw data of the Limit-of-Detection (LOD) experiments of two LAMP assays via fluorescence monitoring are summarized. For the LOD experiments the two remaining LAMP assays were tested using genomic DNA from three P. aeruginosa strains (type strain ATCC 10145, clinical isolate 1, environmental isolate 1). Two-fold serial dilutions were used for all three strains and each concentration was measured in 12 replicate reactions. The LOD of the first LAMP assay was determined with dilutions of the ATCC 10145 type strain ranging from 5.76x10^4 to 7.03 gc/rxt, the clinical isolate 1 from 9.60x10^2 to 1.88 gc/rxt and the environmental strain 1 from 2.80x10^3 to 2.73 gc/rxt. As for the second LAMP assay, ATCC 10145 was measured in 2-fold dilutions from 7.20x10^3 to 7.03 gc/rxt, the clinical isolate 1 from 9.60x10^2 to 0.94 gc/rxt and the environmental strain 1 from 1.40x10^3 to 0.68 gc/rxt.